Purity - ChromScience & Technology

CromSil™ Purity HPLC Columns

(Powered by IsoPore™ silica platform)

Sharper Peaks. Reliable Performance. Built for Precision.

Overview

CromSil™ Purity is Chrom Science & Technology’s flagship range of fully porous HPLC columns, built to deliver exceptional chromatographic performance across the pharmaceutical, biotechnology, food safety, chemical, and environmental sectors.

At the heart of every CromSil™ Purity column lies our proprietary IsoPore™ silica platform—a next-generation engineered particle designed for uniformity, mechanical integrity, and enhanced mass transfer. By combining this advanced silica with high-purity materials and chemically robust bonding techniques, CromSil™ Purity delivers unmatched separation efficiency, peak symmetry, and reproducibility, even under the most demanding conditions.

🧪 What is IsoPore™ Technology?

IsoPore™ is Chrom Science & Technology’s proprietary silica architecture, developed through a precisely controlled manufacturing process that produces fully porous particles with no internal voids and a homogeneous pore network. Unlike conventional porous particles that may suffer from inconsistencies and void formations during synthesis, IsoPore™ ensures:

Consistent and reproducible flow paths for analytes
Faster analyte diffusion and sharper peak formation
Minimal band broadening and peak tailing
High structural rigidity for long column lifetime

The result is cleaner, faster, and more reliable separations, making it ideal for both routine and complex analytical challenges.

⚙️ High-Purity, High-Performance Materials

The silica used in CromSil™ Purity columns is ≥99.99% pure, minimizing trace metal content and secondary interactions with acidic or basic analytes. This ultra-clean surface chemistry—paired with advanced endcapping techniques—provides:

Reduced baseline noise
Improved signal-to-noise ratio
Superior peak shape for both charged and neutral compounds
Stability across a wide pH range (typically pH 2–10, with select phases supporting up to pH 12)

📐 Technical Specifications

Parameter	Details / Options
Particle Sizes	1.7 µm, 1.9 µm, 3 µm, 5 µm, 10 µm
Pore Sizes	100 Å (small molecules), 300 Å (peptides), 1000 Å (proteins)
Silica Purity	≥ 99.99%
Surface Area	~300 m²/g (dependent on pore size)
Pore Volume	~0.65 cm³/g
Chemistries Available	C18, C8, C4, C1 (TMS), Aqua, Biphenyl, PFP, Phenyl, Diol, CN, NH₂
Column IDs	2.1 mm, 3.0 mm, 4.0 mm, 4.6 mm
Lengths	30 mm to 300 mm (standard & custom lengths available)

🧠 Key Advantages of CromSil™ Purity

IsoPore™ Uniformity – Elimination of internal voids = enhanced resolution
Sharp Peak Symmetry – Crucial for accurate quantification and impurity profiling
High Loading Capacity – Suitable for both analytical and semi-preparative applications
Excellent Chemical Stability – Ideal for gradient methods and long run sequences
Lot-to-Lot Reproducibility – Rigorously QC-tested to ensure method validation integrity
Wide Selectivity Range – 16+ chemistries for hydrophobic, polar, and aromatic analytes

Applications Across Industries

Pharmaceutical & Biotech

Impurity profiling in APIs and excipients
LC-MS compatible separations of small molecules and peptides
Bioequivalence studies and method validation
Glycopeptide and oligonucleotide analysis

Food & Beverage

Additive detection (e.g. preservatives, artificial sweeteners)
Mycotoxin and pesticide residue analysis
Nutrient profiling and flavor compound separation

Environmental Testing

Trace-level pollutant monitoring
Analysis of persistent organic compounds (POPs)
Soil and groundwater contaminant profiling

Clinical Research

Therapeutic drug monitoring
Metabolite profiling and biomarker discovery
Protein and peptide separation in biological fluids

💬 Why Choose CromSil™ Purity?

Engineered with IsoPore™ Silica for maximum efficiency
Backed by rigorous quality control for reproducibility
Compatible with both HPLC and UHPLC systems
Available in multiple phases and configurations to fit your workflow
Supported by Chrom Science & Technology’s technical expertise and service

Why Choose Chrom Science & Technology’s Fully Porous Columns?

Exceptional Efficiency & Resolution
High surface area silica (300–450 m²/g) and uniform pore distribution ensure sharper peaks and reliable separation of closely eluting compounds.
Advanced Silica Engineering
Monodense, monodisperse particles with no internal voids reduce mass transfer resistance—resulting in excellent peak symmetry and low backpressure.
High Purity & Chemical Stability
Ultra-pure silica (<100 ppb metals) and robust multi-point bonding deliver inertness, consistent performance, and broad pH compatibility (2–10, up to 1–12 for select phases).
High Sample Capacity
Fully porous architecture enables greater analyte loading, ideal for both analytical and preparative workflows.
Outstanding Reproducibility & Durability
Every column undergoes strict quality testing for efficiency and selectivity to ensure batch-to-batch consistency and long-term stability under repeated gradients.

Let’s solve your toughest material challenges together.

Transform challenges into opportunities.