Optimized for Large Biomolecules and Complex Protein Separations
CromSil™ Bio-Genysis columns are precisely engineered to handle the demands of separating large biomolecules including antibodies, therapeutic proteins, enzymes, and other high molecular weight biologics. With two distinct pore sizes (300Å and 1000Å), the Bio-Genysis range ensures effective diffusion, minimal steric hindrance, and optimal retention of complex macromolecules.
✔ 300Å pore: Superior resolution for medium-sized proteins (up to ~150 kDa), monoclonal antibodies, enzymes
✔ 1000Å pore: Ideal for very large proteins, intact antibodies, and biomolecules (>150 kDa)
✔ Consistent and reproducible chromatographic profiles
✔ Enhanced mechanical robustness and low back pressure for UHPLC/HPLC systems
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Chemistry
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Separation Mechanism
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pH Range
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Carbon Load
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Endcapping
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Performance & Benefits
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C18 (USP CODE: L1)
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Strong hydrophobic reversed-phase
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2–10
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High
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Fully endcapped
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Broad selectivity; suitable for peptides, proteins, and small biomolecules
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C18 (XPH) USP CODE: L1)
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Extra phase-hydrophobicity (for strong hydrophobic binding)
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1–12
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Very High
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Fully endcapped
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Enhanced retention for highly hydrophobic proteins and peptides
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C8 (USP CODE: L7)
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Moderate hydrophobic interactions
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2–10
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Moderate
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Fully endcapped
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Faster elution; suitable for mid-hydrophobic peptides and rapid analyses
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C4 (USP CODE: L26)
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Weak hydrophobic interactions
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2–10
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Low to Moderate
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Fully endcapped
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Ideal for protein recovery and separation of large biomolecules
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C1(TMS) USP CODE: L13)
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Minimal hydrophobicity
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2–9
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Very Low
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Fully endcapped
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Used for very weak interactions or hydrophilic compound separations
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C30 (USP CODE: L62)
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Shape-selective hydrophobic interactions
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2–8.5
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High
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Fully endcapped
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Ideal for structural isomers or geometrical peptide separations
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Aqua (C18 Aqua)
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Hydrophilic endcapped reversed-phase
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2–10
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High
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Polar endcapped
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Suitable for polar peptides and bioanalytes; works well with 100% aqueous mobile phases
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Phenyl (USP CODE: L11)
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π–π interactions (aromatic selectivity)
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2–9
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Medium
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Fully endcapped
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Selectivity for aromatic amino acids or peptide fragments with phenyl groups
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Phenyl Hexyl (USP CODE: L11)
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π–π + moderate hydrophobic interaction
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2–9
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Moderate to High
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Fully endcapped
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Improved selectivity for peptides with both aromatic and aliphatic residues
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Phenyl Hexyl+ (USP CODE: L11)
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Enhanced π–π interactions with extended hydrophobic tail
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2–9
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High
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Fully endcapped
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Enhanced retention for complex peptides and post-translational modifications
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Biphenyl USP CODE: L11)
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Dual π–π interaction
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2–8.5
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Medium
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Fully endcapped
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Targeted for polypeptides with aromatic motifs; enhanced separation resolution
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PFP (USP CODE: L43)
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Electron-withdrawing π-system interactions (fluorinated)
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2–8.5
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Moderate
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Fully endcapped
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Excellent for halogenated and phosphorylated peptides; high selectivity in RP-HPLC
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Cyano (CN) (USP CODE: L10)
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Dipole interactions
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3–7.5
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Low
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Partially endcapped
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Polar selectivity; can be used in both RP and NP modes for oligonucleotides or modified biomolecules
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Diol (USP CODE: L20)
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Hydrogen bonding / normal-phase selectivity
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2–8
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N/A
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Not applicable
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Ideal for glycans, sugars, glycopeptides; HILIC applications for oligosaccharides
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Amino USP CODE: L8)
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Weak anion exchange / polar interactions
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3–8
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N/A
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Not applicable
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Dual RP/HILIC or ion-exchange applications; useful for acidic bio-analytes
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Silica (USP CODE: L3)
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Bare silica – normal phase interactions
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2–7
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None
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None
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Retains polar biomolecules; suitable for normal-phase and HILIC separations
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