CromCarb™ Multi

Unlocking Multimodal Separation Mastery

Designed for the complexity of biopharmaceutical molecules, charge variants, and structural isomers — CromCarb™ Multi delivers separation without compromise.

Overview

Complex Analytes Demand Complex Solutions — Meet CromCarb™ Multi

In today’s analytical and biopharmaceutical landscape, simple reverse phase retention is often insufficient.
Separation of charge variants, glycoforms, hydrophobic impurities, or complex biomolecular assemblies requires stationary phases that can exploit multiple interaction mechanisms simultaneously.

CromCarb™ Multi, based on advanced epoxy-activated graphitized carbon microspheres (NanoPak-M technology), enables true multimodal separations — combining hydrophobic, ionic, and affinity interactions in one powerful platform.

With fully customizable ligand attachment, ultra-stable carbon frameworks, and broad pH/temperature compatibility, CromCarb™ Multi empowers scientists to tackle the toughest separation challenges with unprecedented flexibility and precision.

Technology Platform

The Science Behind Multimodal Mastery

CromCarb™ Multi utilizes precision-engineered epoxy-activated carbon microbeads designed to support customizable functionalization:

Epoxy-Activated Surfaces: Facilitate direct covalent attachment of ion-exchange, hydrophobic, or affinity ligands.
All-Carbon Core: Provides unmatched mechanical, chemical, and thermal stability — surviving harsh solvents and pH swings.
Tunable Porosity and Surface Area: Allows fine control over mass transfer, loading capacity, and selectivity.

Unlike polymeric multimodal supports, CromCarb™ Multi combines bioinert behavior, low non-specific binding, and true hydrophobic plus electrostatic capture — in a fully scalable format.

Key Technical Specifications

Versatile Activation: Ready for covalent ligand coupling tailored to separation needs.

Full-Range Solvent and pH Compatibility: Operates in extreme mobile phases without degradation.

Precision Morphology: Uniform spherical packing reduces eddy diffusion and maximizes resolution.

Parameter	Specification
Particle Sizes	5 µm, 10 µm
Surface Chemistry	Epoxy-activated all-carbon
Functionalization	Customizable (IEX, hydrophobic, affinity)
Pore Size	Broadly tunable
pH Range	0–14
Temperature Stability	Up to 200°C
Ligand Attachment Options	Amine, Carboxyl, Hydroxyl, Sulfonate, etc.

Performance Features

Multimodal Retention — Without Multimodal Headaches

Simultaneous Hydrophobic and Ionic Interactions

CromCarb™ Multi enables dual-mode separation of biomolecules based on charge and hydrophobicity — drastically simplifying method development.

Enhanced Selectivity for Complex Analytes

Resolve charge variants, glycoform isoforms, and post-translationally modified biomolecules more cleanly compared to traditional C18 or ion-exchange alone.

Ultra-Stable Across Harsh Mobile Phases

Survives strong salt gradients, organic solvent spikes, and alkaline/acidic cleaning-in-place protocols — ensuring longer column lifetimes.

Scalability from Discovery to Production

Available in analytical, semi-prep, and preparative configurations — ensuring seamless technology transfer.

Application Segments

CromCarb™ Multi in Action

Charge Variant Profiling of mAbs and Biosimilars: Separates minor charge differences cleanly under mixed-mode conditions.
Glycoform Mapping: Distinguishes glycosylated isoforms without the need for multiple orthogonal methods.
Peptide Therapeutics: Simplifies impurity profiling in hydrophobic peptides and modified biologics.
Polymer and Biopolymer Separations: Enables fractionation of synthetic or natural polymers with mixed surface properties.

Example Study Highlight:
Multimodal interaction studies demonstrated that CromCarb™ Multi enabled simultaneous ionic and hydrophobic capture of semaglutide variants under single gradient conditions — outperforming conventional HIC and IEC combinations.

Commercial & Operational Benefits

A New Level of Efficiency, Sustainability, and Flexibility

Reduced Method Complexity: One column for what previously required multiple orthogonal separations.
Lower Consumable Costs: Durable all-carbon architecture reduces replacement frequency by up to 3–5x vs traditional multimodal supports.
Sustainability: Minimizes need for hazardous salt washes; supports mild cleaning-in-place with NaOH or organic solvents.
Accelerated Method Development: Faster optimization due to simultaneous multimodal behavior.

CromCarb™ Bio vs Silica C18 and Polymeric Columns

Feature	CromCarb™ Multi	Silica Multimodal	Polymeric Multimodal
Stability at pH 0–14	✅ Excellent	❌ Poor	⚪ Good
Stability at 200°C	✅ Highly Flexible	⚪ Moderate	⚪ Moderate
Peak Symmetry	✅ Superior	⚪ Moderate	❌ Variable
Column Lifetime	✅ Extended	❌ Shorter	⚪ Moderate

Conclusion: CromCarb™ Multi doesn’t just mimic multimodal performance — it redefines it.

Final Call to Action

Simplify Complex Separations. Accelerate Innovation