CromCarb™ Bio

Unleashing Bioseparation Excellence with All-Carbon Innovation

Advanced bioseparation columns tailored for peptides, proteins, oligonucleotides, and biomacromolecules — powered by 100% graphitized carbon microbeads.

Overview

The Challenge of Modern Bioseparations — Solved by Carbon Science

In today’s fast-evolving biologics and biotherapeutics landscape, traditional silica-based columns often struggle with peptide mapping, protein separations, and oligonucleotide purification — especially under extreme pH and temperature conditions.

CromCarb™ Bio, crafted from next-generation NanoPak-C graphitized carbon microbeads, offers a new bioseparation paradigm:

No phase collapse.
No secondary ion suppression.
Unparalleled stability for even the most fragile biomolecules.

Engineered specifically for peptides, proteins, ADCs, and oligonucleotides, CromCarb™ Bio delivers higher recovery, enhanced reproducibility, and greater method robustness — enabling true next-generation bioseparation workflows.

Technology Platform

100% Pure Carbon Bioseparation — Engineered for Biomolecular Excellence

Built on the precision of all-carbon microsphere technology, CromCarb™ Bio offers structural and chemical properties tailored to the unique needs of biological macromolecules:

Bioinert Surface Chemistry: Prevents protein adsorption and degradation.
Large Tunable Pore Architectures: 300 Å, 500 Å, and 1000 Å options — ideal for larger peptides and proteins.
Extreme pH Tolerance: Operates seamlessly across pH 0–14.
High Thermal Resilience: Up to 200°C without compromising molecular integrity.

Compared to conventional C18 or polymeric columns, CromCarb™ Bio ensures faster recovery, better peak shape, and superior robustness in aggressive mobile phase environments.

Key Technical Specifications

Optimized Pore Sizes: 300 Å for peptides, 500–1000 Å for large proteins and ADCs.
Lower Surface Energy: Enhances recovery and minimizes biomolecule denaturation.
Stable Under Harsh Cleaning: Compatible with alkaline washes (NaOH, ammonia solutions).

Parameter	Specification
Particle Sizes	5 µm, 10 µm
Surface Area	~100–150 m²/g
Pore Size Options	300 Å / 500 Å / 1000 Å
pH Range	0–14
Temperature Stability	Up to 200°C
Bioanalyte Compatibility	Peptides, Proteins, ADCs, Oligonucleotides

Performance Features

Bioseparation Performance, Amplified by Carbon Technology

Exceptional Retention Without Ion-Pairing

CromCarb™ Bio retains peptides and oligonucleotides naturally — without heavy reliance on TFA or ion-pairing reagents.
Supports cleaner mass spectrometry workflows by minimizing background noise.

High Recovery with Minimal Sample Degradation

Bioinert all-carbon surfaces prevent hydrophobic protein aggregation or loss.
Typical recovery rates exceed 90–95% for large peptides and intact proteins.

Broad Analytical Flexibility

Seamlessly adapts to diverse bioseparation methods:
- Peptide mapping
- Intact mass analysis
- Charge variant profiling
- Glycosylation analysis

Sustainable Biochromatography

Columns withstand multiple aggressive cleaning cycles — extending lifetime, reducing replacement frequency.
Ideal for sustainability-focused manufacturing and high-throughput QC labs.

Application Segments

CromCarb™ Bio — Tailored for the Complexity of Biologics

Peptide Mapping and Sequencing: Achieve sharper peaks and improved sequence coverage under alkaline mobile phases.
Intact Monoclonal Antibody (mAb) Analysis: Stable retention of high-molecular-weight mAbs without tailing or peak distortion.
Oligonucleotide Purification: High-resolution separations under aqueous or basic conditions — compatible with desalting workflows.
ADC Characterization: Supports hydrophobic interaction and linker stability analysis.

Example Study Data:
Using CromCarb™ Bio technology (NanoPak-C base), GLP-1 analogs like Semaglutide and Liraglutide were separated under alkaline pH 8.5 with plate counts exceeding 20,000, outperforming silica C18 controls and demonstrating long-term stability.

Commercial & Operational Benefits

Why CromCarb™ Bio Changes the Economics of Bioseparations

Longer Column Lifetimes: Carbon architecture resists alkaline degradation and high-temperature damage.
Higher Analytical Throughput: Faster recovery means faster cycle times — boosting lab efficiency.
Sustainability and Green Chemistry: Reduces reliance on toxic ion-pairing additives; supports alkaline cleaning-in-place (CIP).
Scalable Across Workflows: Available in analytical, flash, semi-prep, and preparative formats.

CromCarb™ Bio vs Silica C18 and Polymeric Columns

Feature	CromCarb™ Bio	Silica C18	Polymeric Columns
Peptide/Protein Recovery	✅ Superior	❌ Moderate	⚪ Moderate
Phase Collapse in Water	❌ None	✅ Possible	⚪ None
Stability in Alkaline pH	✅ Excellent	❌ Poor	✅ Good
MS Compatibility (Low Ion Suppression)	✅ High	❌ Moderate	⚪ Variable
Thermal Stability	✅ Up to 200°C	❌ Up to 80°C	⚪ 100–120°C

Final Call to Action

Experience the Bioseparation Breakthrough You’ve Been Waiting For.