Silica (USP L3)

Silica (USP L3) – Classic Normal-Phase Chromatography

Overview

Silica columns are widely used for traditional normal-phase chromatography, offering strong polar interactions for the separation of polar compounds, lipids, and organic acids. They are the industry standard for adsorption chromatography.

Key Properties

  • Separation Mechanism: Strong adsorption via hydrogen bonding and dipole interactions.
  • pH Range: pH 2–8, best suited for non-aqueous solvents.
  • Carbon Load (100Å Pore Size): Not applicable; relies on surface silanol interactions.
  • Endcapping: Not endcapped, preserving strong adsorption properties.

Performance & Benefits

High Retention of Polar Compounds Ideal for normal-phase separations.

Strong Surface Interactions Enables selective separation of challenging analytes.

Excellent Resolution High efficiency in separating structurally similar molecules.

USP L3 Compliance Meets regulatory standards for adsorption chromatography.

Recommended Application Areas

  • Pharmaceuticals Separation of lipophilic and polar drugs.
  • Food & Beverage Analysis of oils, lipids, and organic acids.
  • Chemical Industry Purification of fine chemicals and intermediates.
ChromScience

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