C4 (USP L26)

C4 (USP L26) – Optimized for Large Biomolecules

Overview

C4 (Butylsilane) is a short-chain reversed-phase column designed for large biomolecules like proteins and peptides. It offers reduced hydrophobic retention, making it ideal for highly hydrophobic proteins and lipid-soluble compounds.

Key Properties

  • Separation Mechanism: Weaker hydrophobic interactions compared to C18 and C8.
  • pH Range: pH 2–9, compatible with acidic and mild basic conditions.
  • Carbon Load (100Å Pore Size): Lower carbon loading to prevent excessive retention.
  • Endcapping: Fully endcapped for improved peak shape in biomolecular separations.

Performance & Benefits

Optimized for Large Molecules Prevents protein denaturation.

Lower Retention for Hydrophobic Compounds Faster elution times.

Better Peak Shape for Biomolecules Minimal secondary interactions.

USP Compliant Suitable for biopharma quality control.

Recommended Application Areas

  • Biopharmaceuticals Peptides, proteins, monoclonal antibodies.
  • Proteomics Separation of denatured proteins.
  • Food & Beverage Lipid and fat analysis.
  • Pharmaceuticals Large hydrophobic drugs and formulations.
ChromScience

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